Intracellular pH determination of pristinamycin-producing Streptomyces pristinaespiralis by image analysis
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چکیده
منابع مشابه
Characterization of the ‘pristinamycin supercluster’ of Streptomyces pristinaespiralis
Pristinamycin, produced by Streptomyces pristinaespiralis Pr11, is a streptogramin antibiotic consisting of two chemically unrelated compounds, pristinamycin I and pristinamycin II. The semi-synthetic derivatives of these compounds are used in human medicine as therapeutic agents against methicillin-resistant Staphylococcus aureus strains. Only the partial sequence of the pristinamycin biosynth...
متن کاملA Complex Signaling Cascade Governs Pristinamycin Biosynthesis in Streptomyces pristinaespiralis.
Pristinamycin production in Streptomyces pristinaespiralis Pr11 is tightly regulated by an interplay between different repressors and activators. A γ-butyrolactone receptor gene (spbR), two TetR repressor genes (papR3 and papR5), three SARP (Streptomyces antibiotic regulatory protein) genes (papR1, papR2, and papR4), and a response regulator gene (papR6) are carried on the large 210-kb pristina...
متن کاملImprovement of pristinamycin I (PI) production in Streptomyces pristinaespiralis by metabolic engineering approaches
Pristinamycin, produced by Streptomyces pristinaespiralis, which is a streptogramin-like antibiotic consisting of two chemically unrelated components: pristinamycin I (PI) and pristinamycin II (PII), shows potent activity against many antibiotic-resistant pathogens. However, so far pristinamycin production titers are still quite low, particularly those of PI. In this study, we constructed a PI ...
متن کاملNitrogen source governs the patterns of growth and pristinamycin production in 'Streptomyces pristinaespiralis'.
Phosphate-limited synthetic culture media were designed to investigate the growth and the pristinamycin production of 'Streptomyces pristinaespiralis' using different nitrogen sources. During balanced growth, either mineral or organic nitrogen sources were readily utilized. However, glutamate and alanine were used as both nitrogen and carbon source, sparing the utilization of the primary carbon...
متن کاملStreptogramin B biosynthesis in Streptomyces pristinaespiralis and Streptomyces virginiae: molecular characterization of the last structural peptide synthetase gene.
Streptomyces pristinaespiralis and S. virginiae both produce closely related hexadepsipeptide antibiotics of the streptogramin B family. Pristinamycins I and virginiamycins S differ only in the fifth incorporated precursor, di(mono)methylated amine and phenylalanine, respectively. By using degenerate oligonucleotide probes derived from internal sequences of the purified S. pristinaespiralis Snb...
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ژورنال
عنوان ژورنال: Microbiology
سال: 2000
ISSN: 1350-0872,1465-2080
DOI: 10.1099/00221287-146-10-2671